Journals Information
Universal Journal of Microbiology Research(CEASE PUBLICATION) Vol. 9(1), pp. 1 - 7
DOI: 10.13189/ujmr.2021.090101
Reprint (PDF) (227Kb)
Screening of Clostridium difficile Isolates for Metallo-Resistant Gene and Extended Spectrum Beta-Lactamases
Nafisat A. Kachalla *, Ja鈥檃faru M. Inuwa , Halima Isa , Jude F. Chanji
Department of Microbiology, School of Life Sciences, Modibbo Adama University Yola, Nigeria
ABSTRACT
Clostridium difficile naturally inhabits intestinal tract of animals including humans. Spores of C. difficile are known contaminant in the Environment. Indiscriminate use of antibiotics has led to the emergence of a powerful resistance determinant (known as Metallo resistant genes) in many bacteria worldwide. Multidrug resistant bacteria (MRB) pose threats to health globally. This study aims to determine the distribution of Metallo resistant Clostridium difficile in hospital environment within Yola. A total of 150 surface samples from the hospital wards were collected from Federal Medical Centre, Yola (FMC) and Specialist hospital, Yola (SHY) Adamawa state, Nigeria. Clostridium difficile were recovered by inoculating samples in modified Cycloserine-cefoxitin fructose broth (CCFB) and Cycloserine-cefoxitin fructose agar (CCFA). Screening of isolates for extended spectrum 尾-lactamase (ESBL) and metallo-尾-lactamase enzymes was done by double disc diffusion method and Imipenem-EDTA combined disc test respectively. A total of 18 C. difficile isolates were obtained and found to be extended spectrum 尾-lactamase (ESBL) positive. 16 (88.89 %) of the Clostridium difficile were found to be metallo-尾-lactamase positive. Polymerase chain reaction showed that 11(61.1 %) of the isolates possess bla-IMP metallo resistant gene. This study suggests that multidrug resistant C. difficile with metallo resistant genes are wide spread in hospital settings and there is need for relevant authorities to improve on the sanitization of environment as well as continuous surveillance to hinder C. difficile transmission.
KEYWORDS
C. difficile, Hospital Wards, Metallo Resistant Gene, Extended Beta Lactamase
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Nafisat A. Kachalla , Ja鈥檃faru M. Inuwa , Halima Isa , Jude F. Chanji , "Screening of Clostridium difficile Isolates for Metallo-Resistant Gene and Extended Spectrum Beta-Lactamases," Universal Journal of Microbiology Research(CEASE PUBLICATION), Vol. 9, No. 1, pp. 1 - 7, 2021. DOI: 10.13189/ujmr.2021.090101.
(b). APA Format:
Nafisat A. Kachalla , Ja鈥檃faru M. Inuwa , Halima Isa , Jude F. Chanji (2021). Screening of Clostridium difficile Isolates for Metallo-Resistant Gene and Extended Spectrum Beta-Lactamases. Universal Journal of Microbiology Research(CEASE PUBLICATION), 9(1), 1 - 7. DOI: 10.13189/ujmr.2021.090101.