Journals Information
Advances in Pharmacology and Pharmacy Vol. 9(4), pp. 117 - 126
DOI: 10.13189/app.2021.090405
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Anthelmintic Activity of Nigella sativa against Caenorhabditis elegans
Emine Sen 1, Tugce Ogut 2, Abdullah Olgun 3, Ozgul Kisa 4,*
1 Department of Biochemistry, Faculty of Pharmacy, Altinbas University, Istanbul, Turkey
2 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Altinbas University, Istanbul, Turkey
3 Department of Biochemistry, Faculty of Pharmacy, Istinye University, Istanbul, Turkey
4 Department of Medical Microbiology, Faculty of Medicine, Ufuk University, Ankara, Turkey
ABSTRACT
Increasing resistance against classical anthelmintic drugs makes discovering new anthelmintic compounds from natural plants important. Nigella sativa (N. sativa) is used as a medicinal plant overall the world and is known to have anthelmintic activity. Caenorhabditis elegans (C. elegans), a common cost-effective model organism that is easily maintained, is useful to determine the anthelmintic activity of new compounds derived from natural products. In our study we aimed to evaluate through toxicity assays the nematocidal activity of N. sativa on C. elegans during its larval and adult stages. Different concentrations of N. sativa oil (900, 450 and 270 mg/mL) were tested and toxicity assessments were done under stereomicroscope by counting the number of surviving nematodes. This study showed that N. sativa essential oil significantly decreases survival of C. elegans in both larval and adult stages at 900 mg/mL final concentration. Larval-stage worms were more sensitive to N. sativa essential oil than were adults. We recommend further studies on other effects of N. sativa on C. elegans after removing the toxic compound(s) from the extract. The further discovery of N. sativa essential oil compounds responsible for anthelmintic activity and determination of their mechanisms of toxicity can pave the way toward new medicines.
KEYWORDS
Nigella Sativa, Anthelmintic, Caenorhabditis Elegans, Medicinal Plants, Phytotherapy, Herbal Medicine
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Emine Sen , Tugce Ogut , Abdullah Olgun , Ozgul Kisa , "Anthelmintic Activity of Nigella sativa against Caenorhabditis elegans," Advances in Pharmacology and Pharmacy, Vol. 9, No. 4, pp. 117 - 126, 2021. DOI: 10.13189/app.2021.090405.
(b). APA Format:
Emine Sen , Tugce Ogut , Abdullah Olgun , Ozgul Kisa (2021). Anthelmintic Activity of Nigella sativa against Caenorhabditis elegans. Advances in Pharmacology and Pharmacy, 9(4), 117 - 126. DOI: 10.13189/app.2021.090405.