Journals Information
Universal Journal of Plant Science Vol. 10(1), pp. 26 - 37
DOI: 10.13189/ujps.2023.100102
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Germination and Early Growth of Acacia nubica Benth. In vitro
Kauther Sir El-Khatim Ali 1, H. M. Daffalla 2,*, Magdoleen G. Osman 2, A. M. A. Musa 1
1 Department of Biotechnology, College of Applied and Industrial Sciences, University of Bahri, Khartoum North, 12217, Khartoum, Sudan
2 Department of Pant Cell and Tissue Culture, Commission for Biotechnology and Genetic Engineering, National Center for Research, Khartoum, 11111, Khartoum, Sudan
ABSTRACT
Acacia nubica is a well-adapted shrub to harsh conditions that provides valued socioeconomic and environmental advantages. In vitro culture of A. nubica is critical not only for investigating factors affecting cultivation conditions but also for producing juvenile tissue as a potential explants for various in vitro techniques. This research was aimed to study A. nubica seed germination and seedling growth in vitro in response to varied levels of sodium hypochlorite, sucrose, and Murashige and Skoog (MS) medium. The synchrony, percentage, and rate of germination of A. nubica seed were investigated. The shoot, root, and number of nodes were measured to gauge seedling development. Sodium hypochlorite at 10% resulted in the highest percentage of germination, whereas 5% resulted in a faster and more uniform emergence. Sucrose has various effects on seed emergence and germination depending on the concentrations; 30 g/L elicited the highest germination, while 10 g/L improved seed emergence rate with higher uniformity. Although 录-MS medium produced the highest seed germination, 0-MS strength recorded faster and more synchronized germination. The development of A. nubica seedlings was influenced by sucrose concentrations and MS medium strengths. Sucrose at 20 g/L produced the maximum shoot length and vigor index, 30 g/L produced the maximum number of nodes, and 50 g/L produced the maximum root length and root/shoot ratio. Full MS medium supplemented with 30 g/L was shown to be the most effective in improving all assessed growth parameters. This is the first successful report on the seed germination and seedling growth of A. nubica in vitro.
KEYWORDS
Acacia nubica, Dormancy, Germination, In Vitro, Seedling, Synchronization
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Kauther Sir El-Khatim Ali , H. M. Daffalla , Magdoleen G. Osman , A. M. A. Musa , "Germination and Early Growth of Acacia nubica Benth. In vitro," Universal Journal of Plant Science, Vol. 10, No. 1, pp. 26 - 37, 2023. DOI: 10.13189/ujps.2023.100102.
(b). APA Format:
Kauther Sir El-Khatim Ali , H. M. Daffalla , Magdoleen G. Osman , A. M. A. Musa (2023). Germination and Early Growth of Acacia nubica Benth. In vitro. Universal Journal of Plant Science, 10(1), 26 - 37. DOI: 10.13189/ujps.2023.100102.