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Advances in Zoology and Botany Vol. 11(6), pp. 434 - 442
DOI: 10.13189/azb.2023.110604
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GC-MS Analysis and Antioxidant Study on Methanolic Leaf Extract of Endemic Medicinal Plant Crotalaria paniculata Willd


D. Felix Irudhyaraj *, V. Negasta Smila , S. Janani
Department of Botany, Bishop Heber College, India

ABSTRACT

Medicinal plants have many bioactive components that hold numerous activities against diseases and beneficial effects on human metabolism. The knowledge of these Phytochemical constituents had not yet been explored for many plants which are traditionally used in household treatments. Crotalaria paniculata Willd. is one such medicinal plant that belongs to the family Fabaceae used for skincare diseases by tribal people. The present research work is undertaken to identify the phytochemicals and antioxidant activity of methanol leaf extract of C. paniculate Willd. The ion chromatogram of GC – MS analyses showed that the plant accounts for 20 bioactive compounds and among these compounds Cyclohexasiloxane, dodecamethyl contribute 21.98% and 14-Acetyl-13-methoxypodocarpa-8,11,13-Triene with 15.63 % area percentage. Most of the identified compounds are medicinally important that are used in skincare ointments. The antioxidant assay by DPPH, H2O2 and Ion Chelating assay showed a higher percentage of free radical scavenging activity with IC50 value of 39.28 µg/ml (DPPH assay); 72.68 µg/ml (H2O2 assay) and 54.74 µg /ml (Ion Chelating Assay). On compared with the standard Ascorbic acid the result indicates that the plant compounds have potential as antioxidants and further can be investigated for medicinal application.

KEYWORDS
Phytoconstituents, Antioxidant, Crotalaria paniculata, Ion Chelation

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] D. Felix Irudhyaraj , V. Negasta Smila , S. Janani , "GC-MS Analysis and Antioxidant Study on Methanolic Leaf Extract of Endemic Medicinal Plant Crotalaria paniculata Willd," Advances in Zoology and Botany, Vol. 11, No. 6, pp. 434 - 442, 2023. DOI: 10.13189/azb.2023.110604.

(b). APA Format:
D. Felix Irudhyaraj , V. Negasta Smila , S. Janani (2023). GC-MS Analysis and Antioxidant Study on Methanolic Leaf Extract of Endemic Medicinal Plant Crotalaria paniculata Willd. Advances in Zoology and Botany, 11(6), 434 - 442. DOI: 10.13189/azb.2023.110604.