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Advances in Zoology and Botany Vol. 11(6), pp. 466 - 474
DOI: 10.13189/azb.2023.110607
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DNA Metabarcoding: Simplifying Biodiversity


Namita Goyat *, Arvind Malik , Sukhmeet Singh , Joginder Singh Duhan
Department of Zoology, Chaudhary Devi Lal University, Sirsa, India

ABSTRACT

A potent method for quickly analysing species relationships, species composition, and, in conjunction with trait databases, functional elements of community biodiversity, is DNA metabarcoding. Traditional approaches to assessing species richness and abundance are constrained by taxonomic identification, may harm or destroy habitat, and may rely on techniques that make it difficult to find small or elusive species, making estimations for entire communities unattainable. By using highthroughput sequencing (HTS) technology, this technique can sequence and extract data related to DNA barcodes collected from environment and community samples. An evolving technology called metabarcoding makes use of huge libraries of DNA barcode sequences and the improving throughput of DNA sequencing technologies. Analysis of vertebrate diets was one of DNA metabarcoding's earliest applications, and this technique is still effective for comprehending plant-pollinator relationships. The scope of DNA research is constrained by environmental DNA degradation since, especially in warm, tropical areas, only very little remnants of genetic material are frequently present. DNA metabarcoding is a novel technique that is still under development. As technology develops and protocols become more standardised, it is expected that this method will stay in flux for some time. Metabarcoding, however, is anticipated to become a crucial tool for monitoring ecology and global conservation research as it is improved and used more frequently.

KEYWORDS
DNA Metabarcoding, Biodiversity, Barcode, eDNA, Metabarcoding, HTS

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Namita Goyat , Arvind Malik , Sukhmeet Singh , Joginder Singh Duhan , "DNA Metabarcoding: Simplifying Biodiversity," Advances in Zoology and Botany, Vol. 11, No. 6, pp. 466 - 474, 2023. DOI: 10.13189/azb.2023.110607.

(b). APA Format:
Namita Goyat , Arvind Malik , Sukhmeet Singh , Joginder Singh Duhan (2023). DNA Metabarcoding: Simplifying Biodiversity. Advances in Zoology and Botany, 11(6), 466 - 474. DOI: 10.13189/azb.2023.110607.