Journals Information
Advances in Pharmacology and Pharmacy Vol. 13(4), pp. 549 - 559
DOI: 10.13189/app.2025.130407
Reprint (PDF) (1651Kb)
An Experimental Study Comparing and Contrasting the Analgesic and Antioxidant Properties of Morphine and Ergocalciferol in Albino Mice
Ashish Tale 1,*, Balaji Thakare 2, Shirish Jain 1, Sachin Borikar 1
1 Department of Pharmacology, Rajarshi Shahu College of Pharmacy, Amravati University, India
2 Department of Pharmaceutical Chemistry, Anuradha College of Pharmacy, Amravati University, India
ABSTRACT
Background: The current study aims to investigate the analgesic and antioxidant activity of ergocalciferol. Tissues contain vitamin D receptors that help to maintain homeostasis. The objective of this research is to eliminate the adverse effects of drugs that are currently on the market. Methods: This prospective experimental study employed healthy albino mice with IAEC approval. Mice were divided into five groups such as Control group received normal saline, Standard group received morphine 1mg/kg and test group received ergocalciferol at doses of manner like 0.015, 0.03, and 0.06 mg/kg/mice. After treatment, behavioral analysis was carried out for the assessment of central and peripheral analgesic effects using hot plate and tail flick analgesiometers, tail immersion and acetic acid-induced writhing as a well validated animal model for screening of the analgesic effect of drug in a preclinical laboratory. After the writhing test, all animals were sacrificed and screened for oxidative stress markers and antioxidants. Results: At 30鈥�240 min, the test dose of 0.06 mg/kg notably prolonged response time in the hot plate test and tail flick test. Moreover, in the tail immersion method, ergocalciferol at 0.06 mg/kg notably increases tail elongation reaction time to 30鈥�240 min. In the acetic acid writhing test, ergocalciferol at 0.06 mg/kg dramatically inhibits the writhing behavior in experimental animals. Moreover, test drug ergocalciferol at the dose of 0.03 and 0.06 mg/kg restored SOD and GSH levels and conversely found to reduce elevated LPO levels. Conclusion: Ergocalciferol shows central and peripheral analgesic effects, possibly due to its antioxidant potential. Extensive animal study is needed to understand the mechanistic approach.
KEYWORDS
Ergocalciferol, Hot Plate, Paw Withdrawal, Analgesiometer, Writhing, Tail Immersion Test, Lipid Peroxidase, Catalase, Glutathione, Anagesic
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Ashish Tale , Balaji Thakare , Shirish Jain , Sachin Borikar , "An Experimental Study Comparing and Contrasting the Analgesic and Antioxidant Properties of Morphine and Ergocalciferol in Albino Mice," Advances in Pharmacology and Pharmacy, Vol. 13, No. 4, pp. 549 - 559, 2025. DOI: 10.13189/app.2025.130407.
(b). APA Format:
Ashish Tale , Balaji Thakare , Shirish Jain , Sachin Borikar (2025). An Experimental Study Comparing and Contrasting the Analgesic and Antioxidant Properties of Morphine and Ergocalciferol in Albino Mice. Advances in Pharmacology and Pharmacy, 13(4), 549 - 559. DOI: 10.13189/app.2025.130407.