Journals Information
Universal Journal of Microbiology Research(CEASE PUBLICATION) Vol. 2(3), pp. 45 - 49
DOI: 10.13189/ujmr.2014.020301
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Identification and Characterization of KPC-Producing Klebsiella Pneumoniae in Clinical Isolates
Sara Marchi 1, Ilaria Giovanelli 2, Raffaele Gargiulo 2, Daniela Nozzi 2, Agostino Barozzi 2, Mario Sarti 2, Ramona Iseppi 1, Immacolata Anacarso 1, Carla Sabia 1,*
1 Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy
2 Provincial Laboratory of Clinical Microbiology, S. Agostino-Estense Hospital, Modena, Italy
ABSTRACT
The dissemination of carbapenemase-producing Klebsiella pneumoniae (KPC) strains is an increasing problem worldwide. KPC 脽-lactamases are Ambler class A enzymes mostly plasmid-encoded; their global spread represents a threat to clinical patients care and public health. Between September-December 2012, have been isolated 60 Klebsiella pneumoniae strains that showed a decrease of susceptibility to carbapenems, collected from patients at the Provincial Laboratory of Clinical Microbiology 鈥楽. Agostino-Estense' Hospital (Modena, Italy). Species identification and antimicrobial susceptibility testing were carried out using the Vitek 2 and studied for carbapenemases production using phenotypic confirmatory tests. The presence of carbapenemase genes were confirmed by PCR and sequencing. The localization of beta-lactamase genes was established by conjugation experiments. We reported the emergence of KPC-2-producing Klebsiella pneumoniae isolates PCR and sequencing experiments revealed the presence of blaKPC-2 gene in 56/60 isolates. The KPC-encoding plasmid was analysed by transconjugation experiments and DNA sequencing. The findings indicate the need to supplement surveillance systems based on susceptibility data with the surveillance of resistance mechanisms.
KEYWORDS
KPC, Klebsiella pneumoniae, Molecular Genetics, Antibiotic-Resistance
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Sara Marchi , Ilaria Giovanelli , Raffaele Gargiulo , Daniela Nozzi , Agostino Barozzi , Mario Sarti , Ramona Iseppi , Immacolata Anacarso , Carla Sabia , "Identification and Characterization of KPC-Producing Klebsiella Pneumoniae in Clinical Isolates," Universal Journal of Microbiology Research(CEASE PUBLICATION), Vol. 2, No. 3, pp. 45 - 49, 2014. DOI: 10.13189/ujmr.2014.020301.
(b). APA Format:
Sara Marchi , Ilaria Giovanelli , Raffaele Gargiulo , Daniela Nozzi , Agostino Barozzi , Mario Sarti , Ramona Iseppi , Immacolata Anacarso , Carla Sabia (2014). Identification and Characterization of KPC-Producing Klebsiella Pneumoniae in Clinical Isolates. Universal Journal of Microbiology Research(CEASE PUBLICATION), 2(3), 45 - 49. DOI: 10.13189/ujmr.2014.020301.