Journals Information
International Journal of Cardiovascular and Cerebrovascular Disease(CEASE PUBLICATION) Vol. 2(5), pp. 45 - 50
DOI: 10.13189/ijccd.2014.02051
Reprint (PDF) (1265Kb)
Establishment and Evaluation of Isoproterenol Induced Chronic Heart Failure and Cardiac Remodeling Model in Rats: An Experimental Study
Dan Huang 1, Jianting Ke 1, Anzhu Zhao 2, Zhisheng Yang 2, Diefei Liang 2, Jing Pan 2, Mao Liu 1, Jian Chen 1,*
1 Department of Cardiology, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, P, R, China
2 Department of Clinical Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P, R, China
ABSTRACT
Objective: To assess the efficacy of isoproterenol (ISO) in the establishment of chronic heart failure (CHF) and cardiac remodeling model in rats. Methods: Twelve Wistar rats were randomly divided into two groups: control group (n=3) and model group (n=9). Rats in model group were hypodermic injected with ISO 5mg/kg/d for 10 days. Ultrasonic cardiogram, HE staining锛宨mmunohistochemistry of collagen 鈪� and Masson staining were performed to evaluate the cardiac function and myocardial fibrosis. Besides, ventricular mass/body mass ratio, collagen volume fraction (CVF), perivascular collagen area (PVCA), hydroxyproline (HYP) concentration and relative expression of transforming growth factor 尾1 (TGF-尾1) mRNA were also detected. Results: Compared to the controls, rats in model group had a marked enlargement of cardiac dilatation and reduction of ejection fraction (57.00卤3.61% vs. 44.67卤3.06%, P=0.011). Ventricular mass/body mass ratio (3.60卤0.31 vs. 4.88卤0.34, P=0.020), CVF (5.65卤0.68% vs. 27.62卤4.89%锛孭=0.020), PVCA (11.22卤3.40% vs. 28.50卤4.52%, p=0.001) and HYP level (0.24卤0.08渭g/mg wet weight vs. 0.62卤0.11渭g/mg wet weight, P=0.001) were significantly increased in model group. Remarkably cardiac fibrosis were also observed in the Masson staining and immunohistochemistry. The expression of TGF-尾1 mRNA was increased significantly in the model group (P=0.003). The mortality rate in the model group was 33.3% during the whole procedure. Conclusion: ISO can successfully induce a CHF and cardiac remodeling rat model with a low mortality rate.
KEYWORDS
Isoproterenol, Heart Failure, Cardiac Remodeling, Myocardial Fibrosis
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Dan Huang , Jianting Ke , Anzhu Zhao , Zhisheng Yang , Diefei Liang , Jing Pan , Mao Liu , Jian Chen , "Establishment and Evaluation of Isoproterenol Induced Chronic Heart Failure and Cardiac Remodeling Model in Rats: An Experimental Study," International Journal of Cardiovascular and Cerebrovascular Disease(CEASE PUBLICATION), Vol. 2, No. 5, pp. 45 - 50, 2014. DOI: 10.13189/ijccd.2014.02051.
(b). APA Format:
Dan Huang , Jianting Ke , Anzhu Zhao , Zhisheng Yang , Diefei Liang , Jing Pan , Mao Liu , Jian Chen (2014). Establishment and Evaluation of Isoproterenol Induced Chronic Heart Failure and Cardiac Remodeling Model in Rats: An Experimental Study. International Journal of Cardiovascular and Cerebrovascular Disease(CEASE PUBLICATION), 2(5), 45 - 50. DOI: 10.13189/ijccd.2014.02051.