Journals Information
Advances in Pharmacology and Pharmacy Vol. 3(2), pp. 43 - 51
DOI: 10.13189/app.2015.030203
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A Validated Liquid Chromatography-Tandem Mass Spectrometry Coupled with Liquid-Liquid Extraction for Indacaterol Quantitation in Human Plasma
Wesam G. Ammari 1,*, Zainab Al-Qadhi 1, Mohammad Khalil 2, Rabab Tayyem 2, Samir Qammaz 1, Ghaleb Oriquat 1, Iman A. Basheti 3, Henry Chrystyn 4
1 School of Pharmacy and Medical Sciences, Al-Ahliyya Amman University, Jordan
2 ACDIMA Centre for Bioequivalence and Pharmaceutical Studies, Jordan
3 School of Pharmacy, Applied Sciences University, Jordan
4 School of Applied Sciences, University of Huddersfield, United Kingdom
ABSTRACT
Indacaterol is a recent ultra-long-acting inhaled 尾2-agonist bronchodilator. The current work validated an in-house developed high performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) bioassay for indacaterol in human plasma. Ethyl acetate was used in a liquid-liquid extraction (LLE) method to extract indacaterol from plasma samples. A 200 渭L of the mobile phase (water-methanol (30:70, v/v)) was used to reconstitute the indacaterol dry extract. A 5 渭L was then eluted on a C18 column at a mobile phase flow of 1 mL/min. Formoterol was the internal standard (IS). The MS/MS was the detector. Method validation followed the International Guidelines. Indacaterol and IS were detected at a mass to charge ratio of 393.3 and 345.2, respectively. Validated calibration curves were linear over 0.075 - 100 ng/mL. Method specificity was established in 8 different plasma batches. No matrix effect was observed. The intra-batch accuracy (precision) percentages for the lower limit of quantitation (LLOQ), Low, Mid and High quality control levels were 113.6 (10.8), 104.7 (9.1), 99.8 (7.6) and 102.2 (8.5), respectively. The inter-batch accuracy, recovery, short-term and long-term stability results were accepted. Validation has confirmed a specific, accurate and precise HPLC-MS/MS coupled with a simple and fast LLE for indacaterol in human plasma.
KEYWORDS
HPLC-MS/MS, Plasma, Validation, Liquid-Liquid Extraction, Indacaterol
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Wesam G. Ammari , Zainab Al-Qadhi , Mohammad Khalil , Rabab Tayyem , Samir Qammaz , Ghaleb Oriquat , Iman A. Basheti , Henry Chrystyn , "A Validated Liquid Chromatography-Tandem Mass Spectrometry Coupled with Liquid-Liquid Extraction for Indacaterol Quantitation in Human Plasma," Advances in Pharmacology and Pharmacy, Vol. 3, No. 2, pp. 43 - 51, 2015. DOI: 10.13189/app.2015.030203.
(b). APA Format:
Wesam G. Ammari , Zainab Al-Qadhi , Mohammad Khalil , Rabab Tayyem , Samir Qammaz , Ghaleb Oriquat , Iman A. Basheti , Henry Chrystyn (2015). A Validated Liquid Chromatography-Tandem Mass Spectrometry Coupled with Liquid-Liquid Extraction for Indacaterol Quantitation in Human Plasma. Advances in Pharmacology and Pharmacy, 3(2), 43 - 51. DOI: 10.13189/app.2015.030203.