Journals Information
Universal Journal of Microbiology Research(CEASE PUBLICATION) Vol. 1(2), pp. 15 - 21
DOI: 10.13189/ujmr.2013.010201
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Detection and Characterization of AmpC B-Lactamases in Indian Clinical Isolates of Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca
Tanushree Barua1,*, Malini Shariff2, S.S. Thukral2
1 Food Microbiology & Safety Team, Agri-Foods & Health, AgResearch, Hopkirk Research Institute, Corner University Avenue & Library Road Massey University,Tennent Drive,Palmerston North-4474,New Zealand
2 Present address- Department of Microbiology & Immunology, College of Medicine & Health Sciences, Sultan Qaboos University, Muscat, Oman
ABSTRACT
The aim of the study was to determine the prevalence of AmpC producing E. coli, Klebsiellapneumoniae and Klebsiellaoxytoca and toevaluate and compare four different phenotypic methods viz; Modified Three Dimensional Test, AmpC Disk Test, AmpC Disk Test with Tris-EDTA and Inhibitor based detection test for the detection of AmpC尾-lactamases in these isolates, collected during a period of 2003 to 2009 from various hospitals of Delhi, India. Characterization of these enzymes was carried out by polymerase chain reaction (PCR) as well as by nucleotide sequencing. Phenotypic results showed that Inhibitor based detection test, Modified three dimensional test and AmpC disk test detected 40.8% of E. coli and 34% of Klebsiella spp. to be AmpC producers. However, Inhibitor based detection test was found to be simple, highly specific and sensitive in detecting these enzyme producers. PCR and nucleotide sequencing showed the presence of blaCMY-2 and blaACT-1 genes in all the positive isolates.
KEYWORDS
AmpC 尾-lactamases, Boronic acid, CMY-2, ACT-1
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Tanushree Barua , Malini Shariff , S.S. Thukral , "Detection and Characterization of AmpC B-Lactamases in Indian Clinical Isolates of Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca," Universal Journal of Microbiology Research(CEASE PUBLICATION), Vol. 1, No. 2, pp. 15 - 21, 2013. DOI: 10.13189/ujmr.2013.010201.
(b). APA Format:
Tanushree Barua , Malini Shariff , S.S. Thukral (2013). Detection and Characterization of AmpC B-Lactamases in Indian Clinical Isolates of Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca. Universal Journal of Microbiology Research(CEASE PUBLICATION), 1(2), 15 - 21. DOI: 10.13189/ujmr.2013.010201.