Journals Information
Universal Journal of Microbiology Research(CEASE PUBLICATION) Vol. 4(3), pp. 59 - 65
DOI: 10.13189/ujmr.2016.040301
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Effective in Vitro Synergy of Piperacillin/Tazobactam Plus either Netilmicin or Aztreonam against Metallo-尾-lactamase-producing Pseudomonas Aeruginosa
Wesam Hatem Amer *, Marwa Mohamed Ezzat Abd-Elmonsef
Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Egypt
ABSTRACT
This study was designed to compare the activity of piperacillin/tazobactam (TZP) in combination with either netilmicin (NET) or aztreonam (ATM) against metallo-尾 lactamases (M尾L) Pseudomonas aeruginosa. Out of 46 nosocomial Ps. aeruginosa isolates, 38 (82.6%) isolates were carbapenem-resistant by disc diffusion susceptibility testing. These were then screened by imipenem + EDTA combined disc test for M尾L production. Thirty (78.9%) M尾L-producing isolates detected were further tested for antibiotic synergy against NET-TZP and ATM-TZP combinations by Etest synergy and Etest/agar dilution tests. Neither combination showed antagonism. The synergistic effect of NET-TZP combination was detected in [27 (90%) and 28 (93.3%), respectively] and ATM-TZP combination in [24 (80%) and 25 (83.3%), respectively] of the tested isolates by Etest synergy and Etest/agar dilution tests, respectively. Thus, we have determined the synergistic effect of these two combinations for treating M尾L-producing Ps. aeruginosa. Significance and Impact of the Study: The present study has shown valuable information on the combination treatment of Ps. aeruginosa and recommends their use for treatment of resistant Ps. aeruginosa.
KEYWORDS
Pseudomonas Aeruginosa, MBL, Synergy Tests, Netilmicin, Aztreonam, Piperacillin/Tazobactam
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Wesam Hatem Amer , Marwa Mohamed Ezzat Abd-Elmonsef , "Effective in Vitro Synergy of Piperacillin/Tazobactam Plus either Netilmicin or Aztreonam against Metallo-尾-lactamase-producing Pseudomonas Aeruginosa," Universal Journal of Microbiology Research(CEASE PUBLICATION), Vol. 4, No. 3, pp. 59 - 65, 2016. DOI: 10.13189/ujmr.2016.040301.
(b). APA Format:
Wesam Hatem Amer , Marwa Mohamed Ezzat Abd-Elmonsef (2016). Effective in Vitro Synergy of Piperacillin/Tazobactam Plus either Netilmicin or Aztreonam against Metallo-尾-lactamase-producing Pseudomonas Aeruginosa. Universal Journal of Microbiology Research(CEASE PUBLICATION), 4(3), 59 - 65. DOI: 10.13189/ujmr.2016.040301.