Journals Information
Bioengineering and Bioscience(CEASE PUBLICATION) Vol. 4(5), pp. 76 - 87
DOI: 10.13189/bb.2016.040502
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First Report of Lignin Peroxidase Production from Alternaria alternata ANF238 Isolated from Rotten Wood Sample
Anuja Sharma 1, Neeraj K. Aggarwal 1,*, Anita Yadav 2
1 Department of Microbiology, Kurukshetra University, India
2 Department of Biotechnology, Kurukshetra University, India
ABSTRACT
Lignin peroxidases are considered to be the primary enzyme for oxidative depolymerisation of lignin and are responsible for H2O2-dependent oxidation of both phenolic aromatic substrates and non-phenolic lignin model compounds. The present study was undertaken to study the production of Lignin peroxidase enzyme by Alternaria alternata ANF238 isolated from rotten wood sample using water hyacinth as a lignocellulosic substrate under solid state fermentation. Various production parameters like initial moisture content, various carbon sources, nitrogen sources, inducers and their concentrations were also optimized to further increase the production of this enzyme. Maximum lignin peroxidase yield of 7.6 U/g of dry substrate was obtained with water hyacinth as substrate supplemented with 0.5% mannitol as carbon source, 1% ammonium sulphate as nitrogen source and 3 mM MnSO4 as inducer under optimized conditions. These results indicate that Alternaria alternata ANF238 is a potential candidate for production of lignin peroxidase using solid state fermentation, an enzyme with wide industrial and biotechnological applications. This study also promises effective utilization of water hyacinth as a substrate for lignin peroxidase production. To the best of our knowledge, this is the 铿乺st report on LiP production by Alternaria alternata.
KEYWORDS
Alternaria alternata, Eichhornia crassipes, Lignin Peroxidase, Lignolytic Enzymes
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Anuja Sharma , Neeraj K. Aggarwal , Anita Yadav , "First Report of Lignin Peroxidase Production from Alternaria alternata ANF238 Isolated from Rotten Wood Sample," Bioengineering and Bioscience(CEASE PUBLICATION), Vol. 4, No. 5, pp. 76 - 87, 2016. DOI: 10.13189/bb.2016.040502.
(b). APA Format:
Anuja Sharma , Neeraj K. Aggarwal , Anita Yadav (2016). First Report of Lignin Peroxidase Production from Alternaria alternata ANF238 Isolated from Rotten Wood Sample. Bioengineering and Bioscience(CEASE PUBLICATION), 4(5), 76 - 87. DOI: 10.13189/bb.2016.040502.