Journals Information
Bioengineering and Bioscience(CEASE PUBLICATION) Vol. 4(5), pp. 88 - 94
DOI: 10.13189/bb.2016.040503
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Cellulolytic Potential of Actinomycetes Isolated from Different Habitats
Anita Saini 1, Neeraj K. Aggarwal 1,*, Anita Yadav 2
1 Department of Microbiology, Kurukshetra University, India
2 Department of Biotechnology, Kurukshetra University, India
ABSTRACT
Twenty actinomycete isolates capable of utilizing lignocellulosic biomass were isolated from different samples of agricultural field soil, compost and decaying vegetation. Qualitative screening of all isolates during plate assay method using Congo red showed 17 of them producing a clear zone of hydrolysis around the colonies indicating cellulose hydrolytic potential of the isolates. Quantitative estimation of filter paper (FPase) and carboxymethylcellulase (CMCase) activities exhibited by the positive isolates showed that highest CMCase was produced by NAA2 (0.366 IU/ml), while highest FPase activity of 0.075 FPU/ml was observed in NAA14. All of the isolates showed FPase activities comparable to that shown by C. fimi (0.023 FPU/ml) taken as a standard, confirming the cellulose degradation ability of the isolates.
KEYWORDS
Actinomycetes, Cellulolytic, CMCase, FPase, Habitats
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Anita Saini , Neeraj K. Aggarwal , Anita Yadav , "Cellulolytic Potential of Actinomycetes Isolated from Different Habitats," Bioengineering and Bioscience(CEASE PUBLICATION), Vol. 4, No. 5, pp. 88 - 94, 2016. DOI: 10.13189/bb.2016.040503.
(b). APA Format:
Anita Saini , Neeraj K. Aggarwal , Anita Yadav (2016). Cellulolytic Potential of Actinomycetes Isolated from Different Habitats. Bioengineering and Bioscience(CEASE PUBLICATION), 4(5), 88 - 94. DOI: 10.13189/bb.2016.040503.