Journals Information
Bioengineering and Bioscience(CEASE PUBLICATION) Vol. 4(5), pp. 95 - 99
DOI: 10.13189/bb.2016.040504
Reprint (PDF) (620Kb)
Optimization for Callus Induction and Plant Regeneration from Mature Seeds of Thai Rice Variety: Nam Roo (Oryza sativa L.)
Anurug Poeaim 1,*, Supattra Poeaim 1, Ranyikar Poraha 1, Saengthong Pongjaroenkit 2, Pradit Pongthongkam 3
1 Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand
2 Program of Genetics, Faculty of Science, Maejo University, Chiang Mai, 50290, Thailand
3 Thepstri Rajabhat University, 321 Naraimaharat Road Tambon Talaychubsorn Amphur Muang, Lopburi, 15000, Thailand
ABSTRACT
The aim of this study was to develop a productive protocol for optimum callus induction and regeneration of Thai rice variety: Nam Roo (Oryza sativa L.). Callus was induced on either MS (Murashige and Skoog) or NB (Nitsch and Nitsch) media supplemented with various concentrations of 2,4-D(2,4-dichlorophenoxyacetic acid), 0.5 mg/l NAA (伪-naphthaleneacetic acid), 1 g/l L-proline, 30 g/l sucrose and 2.6 g/l phytagel. Callus from Nam Roo seed gave its maximum mean size (391.49 mm3) and mean weight (0.3412 g.) on NB medium supplemented with 1 mg/l 2, 4-D for 4 weeks. For plant regeneration the callus was cultured on either MS or NB media containing with different concentrations of BAP (6-benzylaminopurine) and phytagel, 0.5 mg/l NAA and 30 g/l sucrose. The highest regeneration frequency (%) was observed from callus grown on MS medium composed of 2 mg/l BAP in 5.2 g/l phytagel. It took 6 weeks for the callus to regenerate into a complete plant. Finally, the plantlets were transferred into the plastic pot.
KEYWORDS
Nam Roo Rice (Oryza sativa L.), Callus Induction, Plant Regeneration
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Anurug Poeaim , Supattra Poeaim , Ranyikar Poraha , Saengthong Pongjaroenkit , Pradit Pongthongkam , "Optimization for Callus Induction and Plant Regeneration from Mature Seeds of Thai Rice Variety: Nam Roo (Oryza sativa L.)," Bioengineering and Bioscience(CEASE PUBLICATION), Vol. 4, No. 5, pp. 95 - 99, 2016. DOI: 10.13189/bb.2016.040504.
(b). APA Format:
Anurug Poeaim , Supattra Poeaim , Ranyikar Poraha , Saengthong Pongjaroenkit , Pradit Pongthongkam (2016). Optimization for Callus Induction and Plant Regeneration from Mature Seeds of Thai Rice Variety: Nam Roo (Oryza sativa L.). Bioengineering and Bioscience(CEASE PUBLICATION), 4(5), 95 - 99. DOI: 10.13189/bb.2016.040504.