Journals Information
Bioengineering and Bioscience(CEASE PUBLICATION) Vol. 5(1), pp. 1 - 6
DOI: 10.13189/bb.2017.050101
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Extracellular Production of Amylase and Protease by Penicillium Purpurogenum BKS9
Bijay Kumar Sethi 1,*, Brahmananda Dikshit 1, Santi Lata Sahoo 2, Chinmay Pradhan 2, Sangeeta Sena 3, Bikash Chandra Behera 4
1 Department of Botany, KIIT Science College, Campus-8, KIIT University, Patia, India
2 Microbiology Research Laboratory, P.G. Department of Botany, Utkal University, India
3 Department of Biotechnology, MITS School of Biotechnology, Utkal University, India
4 Department of Biotechnology, North Orissa University, India
ABSTRACT
In the present study, Penicillium purpurogenum BKS9 was used with different agro waste substrates i.e. starch, wheat bran, soya powder, boiled rice, unboiled rice and milk powder for the production of both amylase and protease by liquid static surface fermentation (LSSF). Among the various substrates tested, wheat bran (WB) was found to be the best substrate for maximum (112.64 U/ml) amylase production whereas soya powder (121.23 U/ml) for production of protease. Immobilization study also revealed that the highest amylase was observed (137.6 U/ml) when wheat bran was used as substrate whereas maximum protease production (130.73 U/ml) with soya seed powder. Maximum biomass production was observed (4.4 卤 0.2 g/50ml) when unboiled rice was taken as a substrate in comparison to other substrate.
KEYWORDS
Biotechnological Agro Food Waste Utilization, Fungi Immobilization, Natural Substrates, Wastes Fermentation
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Bijay Kumar Sethi , Brahmananda Dikshit , Santi Lata Sahoo , Chinmay Pradhan , Sangeeta Sena , Bikash Chandra Behera , "Extracellular Production of Amylase and Protease by Penicillium Purpurogenum BKS9," Bioengineering and Bioscience(CEASE PUBLICATION), Vol. 5, No. 1, pp. 1 - 6, 2017. DOI: 10.13189/bb.2017.050101.
(b). APA Format:
Bijay Kumar Sethi , Brahmananda Dikshit , Santi Lata Sahoo , Chinmay Pradhan , Sangeeta Sena , Bikash Chandra Behera (2017). Extracellular Production of Amylase and Protease by Penicillium Purpurogenum BKS9. Bioengineering and Bioscience(CEASE PUBLICATION), 5(1), 1 - 6. DOI: 10.13189/bb.2017.050101.