Journals Information
Bioengineering and Bioscience(CEASE PUBLICATION) Vol. 1(2), pp. 11 - 16
DOI: 10.13189/bb.2013.010201
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Comparative Expression Pattern of Two Vestigial-Like 2 Genes in Zebrafish
Aline Hamade1,*, Francine Rizk2, Carine Genet3, Xavier Cousin4
1 Laboratoirede Physiopathologies Cellulaires et Mol茅culaires, EDST, Universit茅 Libanaise, Lebanon
2 INRA, UMR 1313 G茅n茅tique Animale et Biologie Int茅grative, Jouy-en-Josas, France
3 Ifremer, Laboratoire d'Ecotoxicologie, Place Gaby Coll, BP 7, 17137 L'Houmeau, France
4 INRA, LPGP, Campus Beaulieu, 35042 Rennes Cedex, France
ABSTRACT
The vestigial Vg gene, initially characterized in Drosophila, encodes a transcription co-factor which is crucial for wings development. Vg binds via its Sd interaction domain (SID) to the Scalloped (Sd) transcription factor and its vertebrate homolog Tef1. Previous studies identified several vertebrate genes sharing high homology with Drosophila Vg SID such as Vgl (for Vg-like), TONDU (also known as Vgl1) or Vito-1 (also known as Vgl2). In order to investigate the role of vestigial-like 2 (vgll2) in zebrafish muscle development, we managed to clone and characterize two zebrafishVgll2 homolog genes, Vgll2a and Vgll2b. Alignment data showed high sequence homology of Vgll2a to vertebrates Vgll2 sequences. In situ hybridization showed that the two investigated Vgll2 genes had a similar expression pattern: they were first detected in adaxial cells (11 hpf), than expanded laterally in somites and at the end of segmentation, both genes were expressed in additional structures including head muscles and fin buds. In addition, different expression patterns of the two genes were observed. Vgll2a was expressed in bronchial arches precursor streams, derived gill muscles and hypothalamic precursors. Vgll2b was expressed in notochord at 14 hpf and regressed following notochord maturation at 18hpf. Furthermore, the genetic regulation of vgll2 genes was analysed using smu mutants and data revealed that both genes are regulated via the Hedgehog signaling pathway.
KEYWORDS
Zebrafish; Daniorerio, Vgll2, Vgl2, Vestigial, Somite, Myogenesis, Skeletal Muscles, Jaws Muscles, Branchial Arches, Gill Muscles, Notochord, Hypothalamus
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Aline Hamade , Francine Rizk , Carine Genet , Xavier Cousin , "Comparative Expression Pattern of Two Vestigial-Like 2 Genes in Zebrafish," Bioengineering and Bioscience(CEASE PUBLICATION), Vol. 1, No. 2, pp. 11 - 16, 2013. DOI: 10.13189/bb.2013.010201.
(b). APA Format:
Aline Hamade , Francine Rizk , Carine Genet , Xavier Cousin (2013). Comparative Expression Pattern of Two Vestigial-Like 2 Genes in Zebrafish. Bioengineering and Bioscience(CEASE PUBLICATION), 1(2), 11 - 16. DOI: 10.13189/bb.2013.010201.