Journals Information
Universal Journal of Microbiology Research(CEASE PUBLICATION) Vol. 5(3), pp. 40 - 48
DOI: 10.13189/ujmr.2017.050302
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Production of Bacteriocin Like Substances as Antipathogenic Metabolites by Staphylococcus warneri Isolated from Healthy Human Skin
Reazul Karim *, Mohammad Nuruddin Mahmud , M. A. Hakim
Department of Microbiology, University of Chittagong, Chittagong-4331, Bangladesh
ABSTRACT
Antibiotic resistance is a serious problem of present world and development of viable alternative is urgent. The research work was designed to mitigate this problem. Different types of bacterial colony were isolated from skin of 30 healthy human and their antipathogenic activity was tested against 9 pathogens. The isolate showed activity against four pathogens- Klebsiella. pneumoniae subsp. pneumoniae, Klebsiella. pneumoniae subsp. ozaenae, Staphylococcus. aureus and Pseudomonas. aeruginosa was identified as Staphylococcus. warneri. Variation was found in optimization of cultural conditions (incubation period, incubation temperature and pH) for the most potent antipathogenic metabolites production. Staphylococcus. warneri showed most potent antipathogenic activity at pH 6 to pH 9, at an incubation period of 24h to 48h and at an incubation temperature of 37℃. Antipathogenic metabolites were then detected as bacteriocin like substances. Variation was observed with bacteriocin activity against different pathogens and was found most effective against Klebsiella. pneumonia subsp. pneumonia and Klebsiella. pneumonia subsp. ozaenae Samples containing bacteriocin like substances showed heat stability up to 80℃ for 60 minutes and up to pH 8 for Klebsiella. pneumoniae subsp. pneumoniae and Klebsiella. Pneumoniae sub sp. ozaenae. Papain treated cell-free supernatant did not show any bacteriocin activity, suggesting that the substances could be antimicrobial peptides. Solvent extraction of bacteriocin was performed by using chloroform where maximum bacteriocin activity was found in interface layer rather than aqueous and organic layer.
KEYWORDS
Antipathogenic Metabolites, Bacteriocin, Antimicrobial Peptide, Normal Microflora
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Reazul Karim , Mohammad Nuruddin Mahmud , M. A. Hakim , "Production of Bacteriocin Like Substances as Antipathogenic Metabolites by Staphylococcus warneri Isolated from Healthy Human Skin," Universal Journal of Microbiology Research(CEASE PUBLICATION), Vol. 5, No. 3, pp. 40 - 48, 2017. DOI: 10.13189/ujmr.2017.050302.
(b). APA Format:
Reazul Karim , Mohammad Nuruddin Mahmud , M. A. Hakim (2017). Production of Bacteriocin Like Substances as Antipathogenic Metabolites by Staphylococcus warneri Isolated from Healthy Human Skin. Universal Journal of Microbiology Research(CEASE PUBLICATION), 5(3), 40 - 48. DOI: 10.13189/ujmr.2017.050302.