Journals Information
Bioengineering and Bioscience(CEASE PUBLICATION) Vol. 6(1), pp. 11 - 15
DOI: 10.13189/bb.2018.060102
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Reversal of Antibiotic Resistance by Phage Resistant Pseudomonas aeruginosa PA01
Sadhana Singh Sagar 1,*, Ram Narain 2, Rajesh Kumar 3, Shilpa Deshpande Kaistha 4
1 Department of Microbiology, Rani Lakshmi Bai Central Agricultural University, Jhansi, 284003, India
2 Department of Biotechnology, Veer Bahadur Singh Purvanchal University, Jaunpur, India
3 Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University (A Central University), Lucknow-226025, India
4 Department of Microbiology, Institute of Bioscience and Biotechnology, Chhatrapati Sahu Ji Maharaj University, Kanpur -208024, India
ABSTRACT
Emergence of antibiotic resistance is a serious cause of concern worldwide. Limitation in the discovery of new antibiotics and emergence of resistant infectious microorganisms has directed the attention of researcher toward the phage therapy. Phages are obligate parasite of bacteria and they are highly specific for their host. Phage therapy is an ideal strategy for the control of such infectious pathogens. Pseudomonas aeruginosa PA01 is multiple drug resistant (MDR) bacteria, biofilm forming nosocomial pathogen. Phage therapy of PA01 was studied using bacteriophage 蠁s1 isolated from Ganga water, India. During this study, we observed PA01 variants that had evolved resistant mechanism against phage 蠁s1 infection. These phage resistance cells showed variation in their pigment production from green to brown and also showed reversion of antibiotic resistance for- erythromycin (ERT), norfloxacin (NRF), kanamycin (KAN), gentamicin (GEN), gatifloxacin (GAT), and imipenem (IMP) class of antibiotics. Biofilm forming ability of prPA01 also decreased by up to 90% and they became more susceptible for imipenem antibiotic as compare to PA01.
KEYWORDS
Pseudomonas aeruginosa, Biofilm, Imipenem, Bacteriophage and TEM
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Sadhana Singh Sagar , Ram Narain , Rajesh Kumar , Shilpa Deshpande Kaistha , "Reversal of Antibiotic Resistance by Phage Resistant Pseudomonas aeruginosa PA01," Bioengineering and Bioscience(CEASE PUBLICATION), Vol. 6, No. 1, pp. 11 - 15, 2018. DOI: 10.13189/bb.2018.060102.
(b). APA Format:
Sadhana Singh Sagar , Ram Narain , Rajesh Kumar , Shilpa Deshpande Kaistha (2018). Reversal of Antibiotic Resistance by Phage Resistant Pseudomonas aeruginosa PA01. Bioengineering and Bioscience(CEASE PUBLICATION), 6(1), 11 - 15. DOI: 10.13189/bb.2018.060102.