Journals Information
Universal Journal of Microbiology Research(CEASE PUBLICATION) Vol. 6(2), pp. 15 - 34
DOI: 10.13189/ujmr.2018.060201
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Revealing Microbial Population Complexity on Hospital Textiles Using DHPLC
Ur拧ka Rozman 1,*, Bo啪ena Kotnik Kevorkijan 2, Maja Rupnik 3,4, Sonja 艩ostar Turk 1,5
1 Faculty of Health Sciences, University of Maribor, 沤itna ulica 15, 2000 Maribor, Slovenia
2 Department of Infectious Disease and Febrile Conditions, University Medical Centre Maribor, Ljubljanska ulica 5, 2000 Maribor, Slovenia
3 National Laboratory of Health, Environment and Food, Prvomajska ulica 1, 2000 Maribor, Slovenia
4 Center of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Jamova cesta 39, 1000 Ljubljana, Slovenia
5 Institute of Engineering Materials and Design, Faculty of Mechanical Engineering, University of Maribor, I Smetanova ulica 17, 2000 Maribor, Slovenia
ABSTRACT
Hospital environment provides an important ecological niche that can serve as a reservoir for potentially pathogenic microorganisms. Hospital textiles together with moisture and heat create the right conditions for growth, dissemination and long-term survival of many microorganisms, which may results in transmission of nosocomial infections. Therefore, understanding of total microbial population on hospital textiles is an important part for assessment of public health risks. In the present work, we tested the suitability of DHPLC for estimation of total microbial population on hospital textiles. All of the identified bacteria belonged to the phyla Actinobacteria, Firmicutes and Proteobacteria and up to 63 bacterial genera/species were identified. Mostly abundant species important in perspective of HCAI were Acinetobacter spp., Corynebacterium spp., Staphylococcus spp., Sphingomonas mucosissima and Stenotrophomonas maltophilia. Microbial diversity on hospital textiles was extensive and partly coincides with the intensive care units bacterial communities and pathogens obtained from clinical samples. This is the first study describing total microbial population complexity on hospital textiles, which are important factors contributing to the hospital microbiome.
KEYWORDS
DHPLC, Healthcare Associated Infections, Hospital Environment, Hospital Textiles, Microbial Population
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Ur拧ka Rozman , Bo啪ena Kotnik Kevorkijan , Maja Rupnik , Sonja 艩ostar Turk , "Revealing Microbial Population Complexity on Hospital Textiles Using DHPLC," Universal Journal of Microbiology Research(CEASE PUBLICATION), Vol. 6, No. 2, pp. 15 - 34, 2018. DOI: 10.13189/ujmr.2018.060201.
(b). APA Format:
Ur拧ka Rozman , Bo啪ena Kotnik Kevorkijan , Maja Rupnik , Sonja 艩ostar Turk (2018). Revealing Microbial Population Complexity on Hospital Textiles Using DHPLC. Universal Journal of Microbiology Research(CEASE PUBLICATION), 6(2), 15 - 34. DOI: 10.13189/ujmr.2018.060201.