Journals Information
Advances in Pharmacology and Pharmacy Vol. 7(3), pp. 39 - 48
DOI: 10.13189/app.2019.070301
Reprint (PDF) (612Kb)
A Novel Integrated (OF-HC-EPM) Approach to Study Anxiety Related Depressive Behavior in Mice Model: A Comparison of Neuro Standards
Mohammad M. Billah 1,*, Md. A. Rayhan 2, Syed A. Yousuf 3, Kashfia Nawrin 1, Johir Rayhan 4, Elmabruk M. Khengari 5
1 Department of Pharmacology, Institute for Pharmaceutical Skill Development and Research, Bangladesh
2 Department of Genetic Engineering and Biotechnology, East West University, Bangladesh
3 Department of Anesthesiology, Bangabandhu Sheikh Mujib Medical University, Bangladesh
4 Department of Pharmacy, Jahangirnagar University, Bangladesh
5 Tripoli Children Hospital, Libya
ABSTRACT
Open Field, Hole Cross and EPM are three widely acceptable experimental methods used to evaluate sedative-anxiolytic potential. The theories behind introducing these fields were to challenge the rodents to a novel environment. However, the behavioral changes caused by these environments often get influenced by rodent's identical neurologic conditions. The major challenges faced by the researchers are variations due to first administration against repeated administration, utilizing same rodent for another experiment but in different time or using different rodent for different experiments. Keeping the drawbacks in consideration, the present study undertook a newly modified (OF-HC-EPM) approach to integrate the experimental fields so as to utilize the same rodents with single oral administration for exposure to different fields which had allowed nullifying the risk of individual and time dependent variance. Anxiolytics, atypical antidepressants, selective serotonin reuptake inhibitors, serotonin-norepinephrine reuptake inhibitors, tricyclic antidepressants as well as the combined anxiolytics and antidepressants were administered to Swiss Albino Mice and their respective behavioral changes were observed. The new approach proved to be an essential tool for evaluating neuropharmacological potentials.
KEYWORDS
Open Field, Hole Cross, Elevated Plus Maze, Square Cross, Grooming, Rearing, Stretch Attend Posture, Freezing
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Mohammad M. Billah , Md. A. Rayhan , Syed A. Yousuf , Kashfia Nawrin , Johir Rayhan , Elmabruk M. Khengari , "A Novel Integrated (OF-HC-EPM) Approach to Study Anxiety Related Depressive Behavior in Mice Model: A Comparison of Neuro Standards," Advances in Pharmacology and Pharmacy, Vol. 7, No. 3, pp. 39 - 48, 2019. DOI: 10.13189/app.2019.070301.
(b). APA Format:
Mohammad M. Billah , Md. A. Rayhan , Syed A. Yousuf , Kashfia Nawrin , Johir Rayhan , Elmabruk M. Khengari (2019). A Novel Integrated (OF-HC-EPM) Approach to Study Anxiety Related Depressive Behavior in Mice Model: A Comparison of Neuro Standards. Advances in Pharmacology and Pharmacy, 7(3), 39 - 48. DOI: 10.13189/app.2019.070301.