Journals Information
Advances in Pharmacology and Pharmacy Vol. 8(2), pp. 19 - 23
DOI: 10.13189/app.2020.080202
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Preclinical Evaluation of Anxiolytic Activity of Adhatoda Vasica Leaf Extract in Swiss Albino Mice
Prabhakar Adake , Kumar Gourav , Mahalaxmi S Petimani *
Yenepoya Medical College, Yenepoya (Deemed to be University), India
ABSTRACT
Objectives: To evaluate the possible anxiolytic activity of methanolic extract of Adhatoda vasica leaves (MEAVL) in Swiss albino mice. Methods: A total of 60 (n=60) mice were used. They were divided into ten groups of six mice each. The dose of 200, 400 and 600 mg/kg MEAVL were chosen after acute toxicity study. First 5 groups were evaluated by Elevated Plus Maze (EPM) and rest 5 group were evaluated by Light and Dark Arena (LDA) model. First group of mice (control) received 0.05ml/10g of Normal Saline, second group (standard) received 1.0 mg/kg Diazepam, third, fourth and fifth group received 200, 400 and 600 mg/kg of MEAVL respectively. Anti-anxiety effect was evaluated 60 min after the oral drug administration. Results: All the results were expressed in mean 卤 SD. One way ANOVA followed by Tukey- Kramer multiple comparison tests were used to compare between groups. In EPM model, the numbers of entries in open arm were 3.50卤1.64, 13.17卤7.44, 7.50卤4.63, 3.67卤1.86 and 4.67卤2.94 respectively noted for 1st to 5th groups respectively for Normal Saline, Diazepam, 200, 400 and 600 mg/kg of MEAVL. Similarly in LDA model, the numbers of entries in dark arena were 12.17卤1.47, 13.17卤2.92, 12.33卤3.01, 7.17卤1.94 and 10.50卤3.08 respectively noted for 6th to 10th group. This result clearly showed that MEAVL-400mg/KG has antianxiety property when compared to diazepam as evidenced by increase in the number of entries in dark arena in LDA model with p< 0.05. In EPM, MEAVL showed increase in the time spent in open arm and decrease in the time spent in closed arm, as well as increase in the number of entries in the open arm but it was not statistically significant (p>0.05). Conclusion: The current study has demonstrated an antianxiety effect of MEAVL in a dose of 400mg/kg in LDA model. However, the study plant must be further evaluated to confirm the molecular basis of the antianxiety property so that it can be helpful for the society in the near future because of its antianxiety property.
KEYWORDS
Adhatoda vasica, Elevated Plus Maze, Light and Dark Arena
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Prabhakar Adake , Kumar Gourav , Mahalaxmi S Petimani , "Preclinical Evaluation of Anxiolytic Activity of Adhatoda Vasica Leaf Extract in Swiss Albino Mice," Advances in Pharmacology and Pharmacy, Vol. 8, No. 2, pp. 19 - 23, 2020. DOI: 10.13189/app.2020.080202.
(b). APA Format:
Prabhakar Adake , Kumar Gourav , Mahalaxmi S Petimani (2020). Preclinical Evaluation of Anxiolytic Activity of Adhatoda Vasica Leaf Extract in Swiss Albino Mice. Advances in Pharmacology and Pharmacy, 8(2), 19 - 23. DOI: 10.13189/app.2020.080202.