Journals Information
Universal Journal of Microbiology Research(CEASE PUBLICATION) Vol. 8(2), pp. 13 - 18
DOI: 10.13189/ujmr.2020.080201
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Mycelial Growth and Biological Control Measures of Botrytis cinerea Isolated from Strawberry Fruit Rot Disease in Bangladesh
Sanjida Sultana , Md. Maniruzzaman Sikder , Md. Sabbir Ahmmed , Aireen Sultana , Nuhu Alam *
Department of Botany, Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
ABSTRACT
Fruit rot disease of strawberry caused by fungus is most economically important disease in Bangladesh, which reduces the production and quality of strawberry at both pre and post-harvest period. Present study was conducted to identify the causal organism of the fruit rot disease of strawberry through classical and molecular techniques and its eco-friendly control measures. A fungal pathogen causing fruit rot was isolated and identified as Botrytis cinerea through classical fungal taxonomy and molecular characterization based on their internal transcribed spacer (ITS) region of ribosomal DNA (rDNA). Sequence analysis showed that 5.8S of rDNA sequences were identical. The reciprocal homologies of the ITS region sequences ranged from 98 to 100%. Experimental results suggested that studied fungus (MH371474.1) was genetically similar with MF521935.1 Botrytis cinerea. Isolated fungus was evaluated on six different culture media and at five different temperature conditions. The optimum mycelial growth of B. cinerea was found on Richard agar medium at 25 ℃ temperature at 7days post inoculation (dpi), while mycelial growth was drastically reduced the temperature 30 ℃ and above. Three Trichoderma species viz., T. ressei, T. harzianum and T. asperellum were used to assess the antagonistic effect on isolated pathogenic fungus in which the mycelial growth of it was inhibited mostly by T. ressei. Experimental results revealed that Trichoderma ressei was the effective antagonistic fungus against Botrytis cinerea for the biological control.
KEYWORDS
Botrytis cinerea, Fungal Biology, Molecular Identification, Strawberry
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Sanjida Sultana , Md. Maniruzzaman Sikder , Md. Sabbir Ahmmed , Aireen Sultana , Nuhu Alam , "Mycelial Growth and Biological Control Measures of Botrytis cinerea Isolated from Strawberry Fruit Rot Disease in Bangladesh," Universal Journal of Microbiology Research(CEASE PUBLICATION), Vol. 8, No. 2, pp. 13 - 18, 2020. DOI: 10.13189/ujmr.2020.080201.
(b). APA Format:
Sanjida Sultana , Md. Maniruzzaman Sikder , Md. Sabbir Ahmmed , Aireen Sultana , Nuhu Alam (2020). Mycelial Growth and Biological Control Measures of Botrytis cinerea Isolated from Strawberry Fruit Rot Disease in Bangladesh. Universal Journal of Microbiology Research(CEASE PUBLICATION), 8(2), 13 - 18. DOI: 10.13189/ujmr.2020.080201.