Journals Information
Universal Journal of Food and Nutrition Science(CEASE PUBLICATION) Vol. 2(2), pp. 27 - 32
DOI: 10.13189/ujfns.2014.020201
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Evaluation of the Cytotoxicity of Cholesterol Oxides in Human Colon Cancer Caco-2 Cells
Antonio Alfonso-Garc铆a铆a , Antonio Cilla *, Reyes Barber谩 , Amparo Alegr
Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Spain
ABSTRACT
The content of cholesterol oxides (COPs) in foods varies between 0.1 and 294.3 mg/g. These oxides are formed by auto-oxidative enzymatic processes promoted by the heat treatment of food and/or to exposition of them to the presence of oxygen and sunlight during storage. Their importance is that they are associated with pathological processes like apoptosis, dyslipidemia and pro-oxidative states, among others. The objective was to evaluate the cytotoxicity, by means MTT assay, of 7-keto cholesterol (7KC), cholestane-triol (Triol), 伪-epoxy cholesterol (伪-epoxy C), 尾-epoxy cholesterol (尾-epoxy C) in differentiated Caco-2 cells at 120渭M for 24, 48 and 72h.Triol was the most cytotoxic COP, with a percentage of cell viability among 3.2 to 3.4 at all times studied. The 尾-epoxy C and 7KC showed similar percentages from 65.4 to 6.9 and 67.4 to 6.5, respectively. The 伪-epoxy C was the least cytotoxic (83.3 to 48.7% of cell viability).COPs in the study conditions, showed a cytotoxic effect on Caco-2 cells due to disruption of the integrity of mitochondrial function, being the most toxic compound the triol.
KEYWORDS
Cholesterol Oxides, Cytotoxicity, Caco-2 Cells
Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Antonio Alfonso-Garc铆a铆a , Antonio Cilla , Reyes Barber谩 , Amparo Alegr , "Evaluation of the Cytotoxicity of Cholesterol Oxides in Human Colon Cancer Caco-2 Cells," Universal Journal of Food and Nutrition Science(CEASE PUBLICATION), Vol. 2, No. 2, pp. 27 - 32, 2014. DOI: 10.13189/ujfns.2014.020201.
(b). APA Format:
Antonio Alfonso-Garc铆a铆a , Antonio Cilla , Reyes Barber谩 , Amparo Alegr (2014). Evaluation of the Cytotoxicity of Cholesterol Oxides in Human Colon Cancer Caco-2 Cells. Universal Journal of Food and Nutrition Science(CEASE PUBLICATION), 2(2), 27 - 32. DOI: 10.13189/ujfns.2014.020201.